Application

Use Anti-HA-Peroxidase, High Affinity for the detection of HA-tagged recombinant proteins using: ELISAWestern blotSince Anti-HA High Affinity is a rat monoclonal, it is possible to use it in conjunction with murine monoclonals for double labeling.

General description

Anti-HA-Peroxidase, High Affinity is a monoclonal antibody to the HA-peptide (clone 3F10), conjugated to peroxidase.

Immunogen

The epitope consists of amino acids 98-106 from the human influenza virus hemagglutinin protein.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Preparation Note

Stabilizers: proteinaceous stabilizersWorking concentration: The working concentration of conjugate depends on application and substrate.The following concentrations should be taken as a guideline: Dot blot: 50 mU/ml ELISA: 25 mU/ml Western blot: 50 mU/ml

Reconstitution

Add 1.0 ml double-distilled water for a final concentration of 25 U/mL.Rehydrate for 10 min prior to use.

Specificity

Anti-HA-Peroxidase, High Affinity (3F10) recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein.This epitope is also recognized in fusion proteins regardless of its position (N-terminal, C-terminal or internal).